|Last appearance||Episode 25|
|Voice actor||Shinichiro Miki (Japanese)|
Sonny Strait (English)
Kyuzo is a nearly silent and mysterious samurai. He is, together with Hyogo, a bodyguard for Ayamaro. In that role, he fights with Kambei, and he later joins the group with the stated intent of saving Kanbei's life so that he can later fight Kanbei to the death. Kyuzo is an incredibly skilled fighter, wielding double blades that fit into one sheath on his back.
He's accidentally killed by Katsushiro when the latter used a gun to protect Kanbei because his sword was broken. Rather than showing anger to Katsushiro or regret of his own life, he instead expresses his anticipation of fighting Kanbei in the after world. He is the sixth samurai.